Haha thanks everyone.
Well Johnny, its hard to say. I am acctually just really stepping into manuel mode (Which this was taken in), so i am constantly changing the settings to get the best picture given the light, type of fish, etc. This picture was taken at 1/50 5.6
A lot of the time i'll just turn on Auto mode and put something over the flash as a diffuser for a quick snap session. If i am sitting there longer i start playing with different manuel settings (Shutter, ISO, etc)
Oh and manuel focus, way to many things inbetween the lens and the object to use auto focus. That is if your camera has it.
Maybe i'll start writing settings/conditions down...or get a program to do it for me.