Ok, I've never used bio spira before, but I finaly found a store who had it. When I bought it, I took it home and used it immediately (last night around 10 pm) This morning, I ran a test, and ammonia levels are around the same since yesterday, and nitrite and nitrate levels are 0. Does the biospira produce results that quickly, or do I need to wait another day? The package said "overnight" The package also did not say to refrigerate... do you think maybe the store didnt refrigerate it, and thats what the problem is? The guy got it for me from behind a counter, so i dont know if it was refrigerated or not...