Bio Spira really works,it is a kind of instant bacteria for your tank.Put it for about aday,then is time for u to add your fish.Like Miss Fishy said,they must be keep in refrigerator if not been used.if you take it out,make sure you put it in when it is cold(very cold)i put mine when it is still a ice.The fish wont eat them i guest,they are so small that they are no where to be seen.There is no need to feed them,they will eat dead plants and the fish faeces(thats what other bacteria do,im not sure about bio-spira).They will live for very long term and reproduce.And arh rememba dont make too much WC about -10%-15% daily for your new tank for a more safety precaution.Then if your tank is stable,then u can make it to 30%-40% WC a week.This is what i do when i set up a tank for my friend and myself,and it all worked well or to say perfectly.Ive use this method and in three days im able to add shrimp in without any problem.hehehe,give it a try...........