How much peat it will take depends on what the buffering capacity (KH) of your water is to begin with. If you have a high KH, it is going to take alot of peat. In cases such as this you would be better making the water in a seperate area first. What many people do is this: take a large plastic garbage can and fill it with water. Fill some nylons with peat and let them soak in the water until you get low PH, low KH water. Then, mix this peat filtered water with your tap water to get the desired results. You might want to drop some airstones in the can as well to provide some aeration and keep a film from forming on the water. If you go this way, just go to your local gardening center and buy a large bag of 100% peat moss (make sure there are no additives in it). You will save a ton of $$$ over using "aquarium peat".