RO water is created by passing water through a semi-permiable membrane -against- its osmotic (concentration) gradient, effectively "squeesing out" the water from all the mineral and organic stuff dissoloved in it. RO water is essencially perfect water. Nothing is in it, absolutely no CO2, no DO (dissoloved oxygen), no hardness, no toxic stuff, no nothing! It don't even taste good, but that's just my personal feeling on it. ;D. RO water is -VERY BAD- for freshwater fishies.
What? How can perfect water be bad for fishies? Notice I said -freshwater- fishes. There are trace elements and minerals that are essential to a fish's health that they can only get through their water. Reverse osmosis takes that out of the water. People use RO water on their -saltwater- aquariums because they are effectively returning all those minerals and trace elements back into the water with their seasalt mix and other reef chemicals. The reefkeepers I know are soooo anal about water purity they'd rather make their own water than even trust dechlorinated tap
The lab I worked at had a huge 200 gallon RO unit that we used to run controls on all our tests. If we needed to run a freshwater test, we had to make synthetic freshwater by adding NaCO3, MgSO4, NaCl, KCl, and some other chemicals in pretty exacting ratios that I don't remember. But fish did die in strait RO water, that much I do know. I don't know how much of basic aquarium salts you would need to add to make RO water safe for freshwater fishes.
Bottled spring water has pluses and disadvantges as well. A plus is that you're getting pretty "clean" water with no chlorines, but you're getting different chemistries between brands as well as possibly between bottles of the same brand. Distilling water also removes salt and minerals because these don't evaporate during a steaming process.
RO units are expensive, and if you're only doing a freshwater tank, probably not worth the investment. A better idea would be to just age your water with arration and dechlorinator overnight before you used it.
~~Colesea