just a quick correction, froogyfox. floors are designed in pounds per square foot. most residences are designed with 40 psf in attics and bedrooms, although I'd venture to guess that most everyroom is designed with a 50 psf load in mind. if your floor is built using 2x lumber and you need to find the allowable load, it's not as easy as just measuring the joist spacing and joist size. you need the overall span of the joists (distance between supports) and also what is very important is the type of lumber. wood comes in many species and each has very different design properties. if you're worried about the weight on your floor, talk to a structural engineer or someone who is familiar with construction. they can probably do some calculations and make some "educated guesses" based on visual evaluations and knowing local builders' preference for lumber. always assume worst case scenario to be on the safe side. and like you said span as many joists as you can and try to keep the tank close to a wall or support. sorry for the quick lesson in wood, but i'd hate to see someone lose their fish to a broken floor.