Melafix is a natural antibiotic and is not supposed to interfere with the biological filter which is why i purchased it. I tested the water today and everything reads normal...no ammonia, no nitrites but very little nitrates. The melafix has done its job. All the fish are normal looking and the cory who had a small ulcer on his side is healed. Everyone is eating well and playing and the clown pleco has taken up residence under his bridge again.I always quarentine new fish though i think i'll wait a week or so before thinking about new ones just to be safe.Now that this 100 gal is under control i now have ammonia in the 55 hex. I always do 1 to 2 20-30% water changes every week while vacumming the gravel. This is a 4 year tank. It houses about 10 platys,4 cories, 4 large angels and a large chocolate albino pleco.It also is planted.I never overfeed. No clue what caused this ammonia spike.Everyone seems fine now. I hate to use ammo-lock as you get positive readings for ammonia after you use it.I have not medicated this tank as i lost the one fish who was sick and the others were fine. Although it was medicated with aquari-sol when this whole fiasco started with the 100 gal.Just a thought... my husband bought me a 100 foot python about 6 weeks ago.Prior to that i used buckets and a syphon. There is no way to dechlor while adding new water except to add as you put the water in. The instructions state that due to the water churning so rapidly it elimiates most of the chlorine. What are your thoughts on this ?